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Biochemistry

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JoVE Journal Biochemistry
Detección y cuantificación del péptido relacionado con el gen de la calcitonina (CGRP) en plasma humano mediante un ensayo inmunoabsorbente modificado ligado a enzimas
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Detección y cuantificación del péptido relacionado con el gen de la calcitonina (CGRP) en plasma humano mediante un ensayo inmunoabsorbente modificado ligado a enzimas

Article DOI: 10.3791/64775-v • 07:14 min • June 16th, 2023
June 16th, 2023 •
1,2, 1,3, 1, 4, 5, 1, 6, 1,3, 1
1Department of Otolaryngology-Head & Neck Surgery, Johns Hopkins University School of Medicine, 2Virginia Commonwealth University School of Medicine, 3Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine, 4Department of Otolaryngology-Head and Neck Surgery, University of Michigan Medical School, 5Department of Otolaryngology-Head and Neck Surgery, The Ohio State University College of Medicine, 6ICTR Clinical Research Core Laboratory, Johns Hopkins University School of Medicine

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Los datos publicados relacionados con las concentraciones de péptidos relacionados con el gen de la calcitonina (CGRP) en el plasma humano son inconsistentes. Estas inconsistencias pueden deberse a la falta de una metodología estandarizada y validada para cuantificar este neuropéptido. Aquí, describimos un protocolo validado de ensayo inmunoabsorbente ligado a enzimas (ELISA) para purificar y cuantificar CGRP en plasma humano.

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Este mes en JoVE Número 196
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