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TACI: Ein ImageJ-Plugin für die 3D-Calcium-Imaging-Analyse

Article DOI: 10.3791/64953-v • 09:39 min • December 16th, 2022
December 16th, 2022 •
1,2, 1, 1, 1,3, 4, 1
1School of Neuroscience, Virginia Polytechnic Institute and State University, 2CMU-Pitt Joint Computational Biology, School of Medicine, University of Pittsburgh, 3Eastern Virginia Medical School, 4Department of Physics, University of Miami

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TrackMate Analysis of Calcium Imaging (TACI) ist ein Open-Source-ImageJ-Plugin für die 3D-Calcium-Imaging-Analyse, das die Bewegung auf der z-Achse untersucht und den Maximalwert jedes Z-Stapels identifiziert, um die Intensität einer Zelle zum entsprechenden Zeitpunkt darzustellen. Es kann Neuronen trennen, die sich in lateraler (x / y) Richtung, aber auf verschiedenen Z-Ebenen überlappen.

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