Name: Kwantificering van de modulatie van de activiteit van het elastase-enzym door middel van colorimetrische analyse (Video) | JoVE Journal
Uploaded: 2025-01-17T13:00:00.000+00:00
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Description: Leer een colorimetrische analyse om elastase-activiteit te kwantificeren met natuurlijke plantenextracten. Deze gevoelige methode overwint kleurinterferentie en versnelt onderzoek naar weefselelasticiteit.

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<meta charset="utf8"></head><body>Het meten van elastasemodulatie: een colorimetrische test voor therapeutische inzichten

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<table><tbody><tr><td>96 Well Cell Culture Plate</td><td>Corning Incorporated</td><td>3599</td><td>Flat bottom with lid, polystyrene</td></tr><tr><td>Cell Imaging Multimode Reader</td><td>Agilent</td><td>BioTek Cytation 1</td><td>Gebruikt met Gen5 software</td></tr><tr><td>Elastase From Porcine Pancreas</td><td>Sigma-Aldrich</td><td>E7885</td><td>CAS 39445-21-1; 25,9 kDa</td></tr><tr><td>Isopropanol 99.5%</td><td>Fisher Scientific</td><td>AC184130010</td><td>CAS 67-63-0; C3H8O; 60.10 g/mol</td></tr><tr><td>N-Succinil-(Ala)3-nitroanilide</td><td>Sigma-Aldrich</td><td>S4760</td><td>CAS 52299-14-6; C19H25N5O8 ; 451.43 g/mol</td></tr><tr><td>pH Meter</td><td>Hach Lange</td><td>sensION+ PH31</td><td>Met magnetische roerder en sensorhouder</td></tr><tr><td>Phenylmethanesulfonyl Fluoride</td><td>Sigma-Aldrich</td><td>P7626</td><td>CAS 329-98-6; C7H7FO2S; 174,19 g/mol</td></tr><tr><td>Tris For Molecular Biology</td><td>PanReac AppliChem</td><td>A2264</td><td>CAS 77-86-1; C4H11NO3; 121,14 g/mol</td></tr></tbody></table>

kwantificering van de modulatie van de activiteit van het elastase-enzym door middel van colorimetrische analyse

Quantifying the Modulation of Elastase Enzyme Activity Through Colorimetric Analysis

Kwantificering van de modulatie van de activiteit van het elastase-enzym door middel van colorimetrische analyse

Elastase, a serine protease, plays an essential role in elastin degradation. Elastin is an extracellular protein that helps maintain tissue elasticity in ...

quantifying-modulation-elastase-enzyme-activity-through-colorimetric

Research

JoVE Journal

Biochemistry

Biochemie

De Protease Fluorescent Detection Kit is ontworpen voor de meting van de protease-activiteit met behulp van fluorometrie. Het is ook geschikt voor de detectie van sporen van protease besmetting. De methode is gebaseerd op de proteolytische hydroysis van een eigen formulering van een FITC-gelabelde caseïne substraat.

Gebruik van de Protease Fluorescerende Detection Kit om Protease activiteit te bepalen

Een protocol om trichothecenen (mycotoxinen van zorg voor de gezondheid van de mens) met behulp van een nieuw ontwikkelde screening methode gebaseerd op een competitief immunochemische methode en een laatste elektrochemische detectie te detecteren is aangetoond.

ELIME (Enzyme Linked Immuno Magnetic Electrochemical) Methode voor Mycotoxine Detection

In this video, we perform zymography, an electrophoretic technique, to analyze the proteolytic activity within biological samples.

Fluorescent Peptide Zymography: A Modified Technique to Detect Protease Activity Using Fluorogenic Substrates in Zymogram Gels

This video demonstrates an in vitro assay to quantify neutrophil serine protease activity in sputum samples. The sample containing secreted protease is incubated with a fluorescent reporter bearing a recognition motif. Cleavage of the motif by the protease enables individual fluorescence emission by the donor and acceptor fluorophore of the reporter, indicating protease activity in the sample.

An In Vitro Assay for Measuring Neutrophil Serine Protease Activity Using a Fluorescent Reporter

This video demonstrates the dye release assay for quantifying the enzymatic activity of antimicrobial proteins. The antimicrobial protein degrades the bacteria's dye-labeled cell wall and releases the dye molecules turning the solution blue. The absorbance of the solution correlates with the enzymatic activity of the antimicrobial protein.

A Dye Release Assay to Quantify Enzymatic Activity of Antimicrobial Proteins

We beschrijven een eenvoudige, kwantitatieve colorimetrische test die specifiek de proteolytische activiteit van humane, muis- of ratten-Granzyme B (GzmB) meet. Dit protocol kan eenvoudig worden aangepast voor het bepalen van de protease-activiteit van andere granuulserineproteasen door de hydrolyse van andere synthetische peptide-substraten met een geschikte herkenningssequentie.

Een colorimetrische test waarmee specifiek Granzyme B proteolytische activiteit: Hydrolyse van Boc-Ala-Ala-Asp-S-Bzl

Hier presenteren we protocollen om snel enzymen te screenen en te karakteriseren op antimicrobiële activiteit. De microslide-diffusietest en de kleurstof-releasetest maken gebruik van doelbacteriële substraten voor kwalitatieve en kwantitatieve evaluatie van enzymatische activiteit.

Kwalitatieve en Kwantitatieve Testen voor detectie en karakterisering van proteïne Antimicrobials

Hier presenteren we een gedetailleerd protocol voor een gewijzigde zymographic techniek waarin fluorescerende peptides worden gebruikt als het afbreekbaar substraat in plaats van de inheemse proteïnen. Elektroforese van biologische monsters in fluorescerende peptide zymograms maakt detectie van een breder scala van proteasen dan vorige zymographic technieken.

Opsporen van Protease-activiteit door fluorescerende Peptide Zymography

De hierin beschreven protocollen bieden een gids om de activiteit van neutrofiele proteasen in humaan sputum te visualiseren en te kwantificeren. De toepassingen van een dergelijke analyse variëren van de evaluatie van ontstekingsremmende behandelingen tot biomarkervalidatie, geneesmiddelenscreening en grote cohort klinische studies.

Monitoring van neutrofiele Elastase en Cathepsine G-activiteit in menselijke Sputummonsters

In dit artikel worden gestandaardiseerde ecotoxicologische methoden gepresenteerd voor de evaluatie van biomarkers in neotropische anura-soorten. In het bijzonder beschrijft dit artikel verschillende methodologieën op verschillende schalen van ecotoxicologische evaluatie, zoals genetisch, cellulair-histologisch, biochemisch, morfologisch en individueel niveau.

Ecotoxicologische methodologieën om biomarkers op verschillende schalen te evalueren bij neotropische anura's

Deze studie presenteert een eenvoudige, gebruiksvriendelijke ratiometrische FRET-test voor de detectie en kwantitatieve beoordeling van DNase-activiteit, en demonstreert de toepassing ervan bij de analyse van een zwakke nuclease.

Beoordeling van DNase-activiteit door ratiometrische fluorescentieresonantie energieoverdracht

Leer een colorimetrische analyse om elastase-activiteit te kwantificeren met natuurlijke plantenextracten. Deze gevoelige methode overwint kleurinterferentie en versnelt onderzoek naar weefselelasticiteit.

Kwantificering van de modulatie van de activiteit van het elastase-enzym door middel van colorimetrische analyse

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