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High Throughput Screening of Fungal Endoglucanase Activity in Escherichia coli

Article DOI: 10.3791/2942-v • 06:16 min • August 13th, 2011
August 13th, 2011 •
1, 2
1Department Of Chemistry and Chemical Engineering, California Institute of Technology, 2Chemistry and Chemical Engineering, California Institute of Technology

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We describe a low cost, high throughput method to screen for fungal endoglucanase activity in E. coli. The method relies on a simple visual readout of substrate degradation, does not require enzyme purification, and is highly scalable. This allows for the rapid screening of large libraries of enzyme variants.

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High Throughput Screening Fungal Endoglucanase Activity Escherichia Coli Cellulase Enzymes Cellulose Hydrolysis Fuel Alcohols Enzymatic Hydrolysis Cellulosic Biomass Renewable Energy Engineer Cellulases Fungal Cellulases Mutant Cellulases Library Genetic Tools For Native Organisms Expressing Enzymes In Heterologous Hosts E. Coli Expression Industrial Fungus H. Jecorina Cellulases Secretion Macrophomina Phaseolina Phanerochaete Chrysosporium High Throughput Screening Congo Red Dye
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