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Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting
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Multiplexed Fluorometric ImmunoAssay Testing Methodology and Troubleshooting

Article DOI: 10.3791/3715-v • 08:06 min • December 12th, 2011
December 12th, 2011 •
1, 1, 1, 1
1Research Animal Diagnostic Services (RADS), Charles River

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Using Luminex Corporation’s xMAP microsphere technology, we have developed the Multiplexed Fluorometric ImmunoAssay (MFIA) for serosurveillance of various laboratory animal species. The MFIA is a suspension microarray where antigen, tissue control or immunoglobulins are covalently linked to color-coded polystyrene microspheres. The MFIA testing method as well as various troubleshooting topics is addressed.

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Multiplexed Fluorometric ImmunoAssay Diagnostic Testing Animal Models Biomedical Research Infectious Agents Viruses Mycoplasma Microorganisms Serology Antibody Responses Serosurveillance Enzyme-linked Immunosorbent Assay (ELISA) Multiplexed Assay Microspheres Color Sets Serum Reagents Disposables Internal Control Beads Tissue Control IgG Anti-test Serum Species Immunoglobulin (αIg)
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