Simultânea de dois fótons In Vivo Imagem de Synaptic Entradas e metas pós-sinápticos no córtex do rato retrospl�ico

Overview

This video demonstrates the craniotomy procedure for chronic imaging of neurons in the mouse retrosplenial cortex using in vivo two-photon microscopy. The method involves the injection of mCherry-expressing adeno-associated virus into the dorsal hippocampus, enabling long-term monitoring of structural plasticity.

Key Study Components

Area of Science

• Neuroscience
• Imaging Techniques
• Structural Plasticity

Background

• Chronic imaging techniques are essential for studying neuronal changes over time.
• The retrosplenial cortex is a critical area for understanding spatial navigation and memory.
• Two-photon microscopy allows for high-resolution imaging of neuronal structures.
• Adeno-associated viruses are commonly used for gene delivery in neuroscience research.

Purpose of Study

• To visualize morphological changes in neuronal structures over time.
• To explore the role of the retrosplenial cortex in experience-dependent plasticity.
• To establish a reliable method for chronic imaging in live animals.

Methods Used

• Craniotomy procedure for access to the retrosplenial cortex.
• In vivo two-photon microscopy for imaging neuronal activity.
• Injection of mCherry-expressing adeno-associated virus into the dorsal hippocampus.
• Use of Thy1-GFP transgenic mice for visualization of specific neurons.

Main Results

• Successful implantation of a cranial window for imaging.
• Long-term monitoring of structural changes in the retrosplenial cortex.
• Demonstration of experience-dependent plasticity in neuronal structures.
• Validation of the method for future studies in live animal models.

Conclusions

• The craniotomy and imaging techniques provide valuable insights into neuronal plasticity.
• This approach can be applied to various studies involving the retrosplenial cortex.
• Future research can build on these methods to explore other brain regions.

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