JoVE Journal
Medicine
Medicine
É necessário ter uma assinatura JoVE para assistir este
Capítulos
Resumo
Three-dimensional culture in alginate beads, due to its simplicity and reproducibility, was chosen to maintain the pituitary adenoma cells. The procedures included an initial enzymatic digestion and mechanical dissociation of the tumor tissue, and the subsequent cell suspension was encapsulated in alginate beads.