Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins

Jacob O'Brien; Heyam Hayder; Chun Peng

doi:10.3791/54719

Automated Quantificação e análise dos procedimentos celular contagem utilizando ImageJ Plugins

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Biology

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Automated Quantification and Analysis of Cell Counting Procedures Using ImageJ Plugins

Automated Quantificação e análise dos procedimentos celular contagem utilizando ImageJ Plugins

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11:01 min

November 17, 2016

DOI: 10.3791/54719-v

Jacob O'Brien¹, Heyam Hayder¹, Chun Peng¹

¹Department of Biology,York University

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Overview

This paper presents new open-source ImageJ plugins, Cell Concentration Calculator and migration assay Counter, for quantifying hemocytometer and migration/invasion micrographs. It also details image acquisition and calibration protocols, along with the input requirements for the plugins.

Key Study Components

Area of Science

Neuroscience
Cell Biology
Image Analysis

Background

Cell counting is essential for various biological assays.
Accurate quantification aids in understanding cell behavior.
Digital analysis tools can enhance counting efficiency.
Open-source tools promote accessibility in research.

Purpose of Study

To provide a method for quick and accurate cell counts.
To facilitate quantification in in vitro motility assays.
To describe the use of ImageJ plugins for cell analysis.

Methods Used

Utilization of ImageJ plugins for cell counting.
Image acquisition using a microscope with phase contrast filters.
Calibration protocols for accurate measurements.
Setting default image capture settings in microscope software.

Main Results

Demonstrated effectiveness of the plugins in cell quantification.
Provided detailed protocols for image acquisition.
Highlighted the advantages of digital analysis tools.
Showed how to meet input requirements for the plugins.

Conclusions

The new plugins significantly improve cell counting accuracy.
They offer a user-friendly approach for researchers.
Digital tools can streamline experimental workflows.

Frequently Asked Questions

What are the main advantages of using the new plugins?

The plugins provide fast and accurate cell counts with multiple analysis tools.

How do I set up the microscope for image acquisition?

Set the illumination to maximum and use the 4x objective with phase contrast filters.

Are the plugins suitable for all types of cell assays?

Yes, they can be used for various in vitro assays, including migration and invasion assays.

Can I access the plugins for free?

Yes, the plugins are open-source and available for free.

What is the importance of calibration in this protocol?

Calibration ensures accurate measurements and reliable results in cell counting.

Is training required to use the plugins effectively?

Basic familiarity with ImageJ is helpful, but the plugins are designed to be user-friendly.

Este artigo descreve a quantificação de hemocitômetro e micrografias de migração/invasão por meio de dois novos plug-ins ImageJ de código aberto: Cell Concentration Calculator e migration assay Counter. Além disso, descreve os protocolos de aquisição e calibração de imagens, bem como discute em detalhes os requisitos de entrada dos plug-ins.

O objetivo geral deste protocolo é usar ferramentas de análise digital para fazer rapidamente contagens precisas de células em uma suspensão ou em uma migração em uma membrana de ensaio de invasão. Este protocolo pode ajudar os pesquisadores a quantificar o número de células necessárias para técnicas comuns e ensaios de motilidade celular in vitro. A principal vantagem desse método é que ele fornece contagens de células rápidas e precisas, incluindo vários filtros e ferramentas de análise.

Para começar, defina a iluminação do microscópio para o máximo. Mude para a objetiva 4x e certifique-se de que os filtros de contraste de fase estejam em uso. Em seguida, no software do microscópio, defina as configurações de captura de imagem para seus valores padrão.

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