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Spatial and Temporal Control of T Cell Activation Using a Photoactivatable Agonist

Article DOI: 10.3791/56655-v • 07:48 min • April 25th, 2018
April 25th, 2018 •
1,2, 1
1Immunology Program, Memorial Sloan-Kettering Cancer Center, 2Weill-Cornell Graduate School of Medical Sciences

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This protocol describes an imaging-based method to activate T lymphocytes using photoactivatable peptide-MHC, enabling precise spatiotemporal control of T cell activation.

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Photoactivatable Agonist T Cell Activation Immune Cell Signal Transduction Spatial Control Temporal Control T Cell Receptor Activation Biotinylated Poly-L-lysine Blocking Buffer Streptavidin HBS
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