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JoVE Journal Biochemistry
Beoordeling van cellulaire oxidatie met behulp van een subcellulair compartiment-specifieke Redox-gevoelige groene fluorescerende eiwitten
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Beoordeling van cellulaire oxidatie met behulp van een subcellulair compartiment-specifieke Redox-gevoelige groene fluorescerende eiwitten

Article DOI: 10.3791/61229-v • 06:10 min • June 18th, 2020
June 18th, 2020 •
1,2, 1, 1, 1
1Division of Radiation Health, Pharmaceutical Sciences, University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Ege University

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Dit protocol beschrijft de beoordeling van de subcellulaire compartimentspecifieke redox-status in de cel. Een redoxgevoelige fluorescerende sonde maakt een handige ratiometrische analyse in intacte cellen mogelijk.

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Biochemie redoxgevoelig groen fluorescerend eiwit (roGFP) cytosolic roGFP cysteïne/cystine verhouding live celbeeldvorming flow cytometrie oxidatie/reductie
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