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JoVE Journal Neuroscience
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Hippocampal Neuronal Cultures to Detect and Study New Pathogenic Antibodies Involved in Autoimmune Encephalitis

Article DOI: 10.3791/63829-v • 08:20 min • June 2nd, 2022
June 2nd, 2022 •
1, 2, 1, 1,2
1ICFO-Institut de Ciències Fotòniques, The Barcelona Institute of Science and Technology, 2Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Hospital Clínic, Universitat de Barcelona

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Autoimmune encephalitis is a new category of antibody-mediated diseases of the central nervous system. Hippocampal neurons can be used to discover and characterize these antibodies. This article provides a protocol for primary cell culture and immunostaining to determine autoantibodies in the serum and cerebrospinal fluid of patients.

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Hippocampal Neuronal Cultures Pathogenic Antibodies Autoimmune Encephalitis Reactivity Surface Antigens Intracellular Antigens Neuronal Surface Proteins Patient Outcomes Monotherapies Embryo Head Curved Forceps Straight Forceps Surgery Scissors Dissecting Sc
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