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JoVE Journal Neuroscience
Ganzhirn-Einzelzellbildgebung und Analyse intakter neonataler Mausgehirne mittels MRT, Gewebereinigung und Lichtblattmikroskopie
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Ganzhirn-Einzelzellbildgebung und Analyse intakter neonataler Mausgehirne mittels MRT, Gewebereinigung und Lichtblattmikroskopie

Article DOI: 10.3791/64096-v • 08:49 min • August 1st, 2022
August 1st, 2022 •
*1,2, *1,2, 1,2, 1,2, 3, 3,7, 7, 4,5,6, 4,5,6, 3, 4,5,6, 1,2
1UNC Neuroscience Center, University of North Carolina, Chapel Hill, 2Department of Genetics, University of North Carolina, Chapel Hill, 3Department of Psychiatry, University of North Carolina, Chapel Hill, 4Center for Animal Magnetic Resonance Imaging, The University of North Carolina at Chapel Hill, 5Biomedical Research Imaging Center, The University of North Carolina at Chapel Hill, 6Department of Neurology, The University of North Carolina at Chapel Hill, 7Department of Computer Science, The University of North Carolina at Greensboro
* These authors contributed equally

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Dieses Protokoll beschreibt Methoden zur Durchführung von Magnetresonanztomographie, Clearing und Immunmarkierung intakter Mausgehirne mit iDISCO+, gefolgt von einer detaillierten Beschreibung der Bildgebung mittels Lichtblattmikroskopie und nachgeschalteten Analysen mit NuMorph.

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Neuroscience Ausgabe 186
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