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Eine einfache Tischfiltrationsmethode zur Isolierung kleiner extrazellulärer Vesikel aus humanen mesenchymalen Stammzellen

Article DOI: 10.3791/64106-v • 09:10 min • June 23rd, 2022
June 23rd, 2022 •
1,2, 3, 4, 2, 5, 4, 1, 6,7
1Centre for Tissue Engineering and Regenerative Medicine, Universiti Kebangsaan Malaysia Medical Centre, 2My CytoHealth Sdn. Bhd., 3School of Biosciences, Faculty of Health and Medical Sciences, Taylor's University, 4School of Pharmacy, Monash University Malaysia, 5Haematology Unit, Cancer Research Centre, Institute for Medical Research (IMR), National Institutes of Health (NIH), Ministry of Health Malaysia, 6School of Pharmacy, Faculty of Health & Medical Sciences, Taylor's University, 7Centre for Drug Discovery and Molecular Pharmacology (CDDMP), Faculty of Health and Medical Sciences, Taylor's University

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Dieses Protokoll demonstriert, wie die kleinen extrazellulären Vesikel (hUC-MSC-sEVs) von humanen Nabelschnurstammzellen (hUC-MSC-sEVs) mit einer einfachen Laborumgebung isoliert werden können. Die Größenverteilung, Proteinkonzentration, sEVs-Marker und Morphologie isolierter hUC-MSC-sEVs werden durch Nanopartikel-Tracking-Analyse, BCA-Protein-Assay, Western Blot bzw. Transmissionselektronenmikroskop charakterisiert.

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