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JoVE Journal Bioengineering
使用CRISPR SunTag-p65-HSF1激活系统研究米色脂肪生物学和代谢
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使用CRISPR SunTag-p65-HSF1激活系统研究米色脂肪生物学和代谢

Article DOI: 10.3791/64849-v • 09:52 min • January 6th, 2023
January 6th, 2023 •
*1, *1, 1,2
1Obesity and Comorbidities Research Center, State University of Campinas, 2Department of Structural and Functional Biology, Institute of Biology, State University of Campinas
* These authors contributed equally

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该协议提出了在脂肪细胞（AdipoSPH）中使用CRISPR SunTag-p65-HSF1（SPH）作为腺相关病毒（AAV）的替代策略来研究米色脂肪生物学。 体内 注射靶向内源性Prdm16基因的携带AAV的sgRNA足以诱导米色脂肪发育并增强产热基因程序。

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本月在JoVE上，第191期，脂肪组织，产热，新陈代谢，CRISPR
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