Name: Um método HPLC-QQQ-MS eficiente e rápido para a quantificação de alcaloides tropanos em plantas medicinais (Vídeo) | JoVE Journal
Uploaded: 2026-06-12T13:00:00.000+00:00
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Description: Aprenda a quantificar alcaloides tropanos em Anisodus tanguticus usando cromatografia líquida de alta performance acoplada à espectrometria de massa em tandem. Este método rápido e sensível supera as limitações do HPLC convencional.

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<table><tbody><tr><td>Agilent MassHunter software</td><td>Agilent Technologies</td><td></td><td></td></tr><tr><td>Agilent ZORBAX SB-C18 column</td><td>Agilent Technologies</td><td>880975-902</td><td>C18 reversed-phase column, 4.6 &times; 250 mm, 5 &mu;m</td></tr><tr><td>Anisodamine hydrobromide</td><td>Chengdu glip Biotechnology Co., Ltd</td><td>101-31-5</td><td>HPLC>98%</td></tr><tr><td>Formic Acid</td><td>Sigma-Aldrich</td><td>5.33002</td><td></td></tr><tr><td>freeze dryer</td><td>EYELA, Tokyo, Japan</td><td>FDU-2110</td><td></td></tr><tr><td>High-speed universal grinder</td><td>Taisite Instrument Co., Ltd., Tianjin, China</td><td>6010210100</td><td>FW-100,24,000 rpm, 100 g capacity, 80 mesh</td></tr><tr><td>Hyoscyamine</td><td>Chengdu glip Biotechnology Co., Ltd</td><td>55449-49-5</td><td>HPLC>98%</td></tr><tr><td>Methanol</td><td>Thermo Fisher Scientific</td><td>022909.K2</td><td></td></tr><tr><td>Microporous Membrane 0.22&mu;m</td><td>Millipore</td><td>SLGV033RB</td><td></td></tr><tr><td>Milli-Q Direct 8 water system</td><td>MilliporeSigma, Burlington, MA, USA</td><td>ZRQSVP0JP</td><td></td></tr><tr><td>Scopolamine hydrobromide</td><td>Chengdu glip Biotechnology Co., Ltd</td><td>114-49-8</td><td>HPLC>98%</td></tr></tbody></table>

um método hplc-qqq-ms eficiente e rápido para a quantificação de alcaloides tropanos em plantas medicinais

An Efficient and Rapid HPLC-QQQ-MS Method for the Quantitation of Tropane Alkaloids in Medicinal Plants

Separação cromatográfica e identificação de TAs HPLC-QQQ-MS Um cromatograma típico de MRM obtido da análise de uma mistura padrão e um extrato de raiz de A. tanguticus é apresentado nas Figuras 3 e 4. Sob as condições otimizadas do HPLC, os três alcaloides tropanos-alvo: hiosciamina, anisodamina e escopolamina, foram separados com sucesso em 20 minutos, demonstrando a alta eficiência do método desenv...

Um método HPLC-QQQ-MS eficiente e rápido para a quantificação de alcaloides tropanos em plantas medicinais

Anisodus tanguticus is an important Tibetan medicinal plant. The tropane alkaloids (TAs), such as hyoscyamine, anisodamine, and scopolamine, exhibit ...

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Research

JoVE Journal

Chemistry

Química

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Apresentamos um método para anotação e classificação rápida baseada em espectrometria de massa (MS) / espectrometria de massa (MS) de alcalóides tropanos, útil tanto para a desreplicação preliminar de amostras contendo tropano quanto para a descoberta de novos alcalóides para isolamento.

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Este protocolo apresenta um método validado de cromatografia líquida-mobilidade iónica e espectrometria de massa de alta resolução para determinar a presença de alcaloides da cravagem nos alimentos, em conformidade com o Regulamento (UE) 2023/915 da Comissão, recentemente publicado.

Detecção de Alcalóides Ergot Regulados em Matrizes de Alimentos por Cromatografia Líquida - Espectrometria de Mobilidade Iônica Aprisionada - Espectrometria de Massa por Tempo de Voo

Detalhes do presente Protocolo, os passos importantes necessários para a bioconjugação de uma cisteína contendo proteína para uma maleimida, incluindo a purificação de reagentes, condições de reacção, purificação e caracterização de bioconjugados bioconjugado.

Síntese de Bioconjugados de proteínas<em&gt; via</em&gt; Química cisteína-maleimida

Descrevemos a polimerização por inserção catalítica do ácido 5-norborneno-2-carboxílico e 5-vinil-2-norborneno para formar polímeros funcionais com uma temperatura de transição vítrea muito alta.

Um protocolo simples e eficiente para a polimerização por inserção catalítica de norbornenos funcionais

Um procedimento pormenorizado para a síntese de uma azida marcado com 125I e a radiomarcação de dibenzocyclooctyne (DBCO) -Grupo-conjugados, nanopartículas de ouro de 13 nm de tamanho, utilizando uma reacção de clique sem cobre é descrito.

Um protocolo otimizado para a radiomarcação eficiente de nanopartículas de ouro usando um<sup&gt; 125</sup&gt; Marcado com Eu Azida grupo prostético

Aprenda a quantificar alcaloides tropanos em Anisodus tanguticus usando cromatografia líquida de alta performance acoplada à espectrometria de massa em tandem. Este método rápido e sensível supera as limitações do HPLC convencional.

Um método HPLC-QQQ-MS eficiente e rápido para a quantificação de alcaloides tropanos em plantas medicinais

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