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October 13, 2015
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Effector translocation into host cells via a type III secretion system is a common virulence strategy among gram-negative bacteria. A beta-lactamase effector fusion based assay for quantitative analysis of translocation was applied. In Yersinia infected cells, conversion of a FRET reporter by the beta-lactamase is monitored using laser scanning microscopy.
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Wolters, M., Zobiak, B., Nauth, T., Aepfelbacher, M. Analysis of Yersinia enterocolitica Effector Translocation into Host Cells Using Beta-lactamase Effector Fusions. J. Vis. Exp. (104), e53115, doi:10.3791/53115 (2015).
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