小鼠强迫游泳实验
Summary
强迫游泳测试是在啮齿类动物实验的方法来评估潜在的抗抑郁疗效验证。实验动物被放置在一缸水和逃避相关的流动性行为，量化。共同的这个测试鼠标版的程序进行了阐述。
Abstract
强迫游泳试验是一种啮齿动物行为学测试，用于评价抗抑郁药物，抗抑郁疗效的新化合物，实验操作，目的是在渲染或防止国家像抑郁。小鼠被放置在透明水箱，装满水，并测量其逃避有关流动性的行为是负有不可推卸的。强迫游泳测试是直接进行可靠的，它需要最少的专用设备。强迫游泳试验的成功实施需要遵守一定的程序细节，并尽量减少不必要的压力的老鼠。在协议的描述和影随行中，我们解释了如何进行鼠标本次测试的版本，强调对潜在的隐患，可能危害结果的解释，以及如何避免它们。此外，我们解释了如何在测试中表现出来的行为评估。
Protocol
1。材料与方法 1.1。水箱 （30厘米高x 20厘米直径）的圆柱型坦克所需的小鼠强迫游泳实验（FST）的在我们的实验室，是透明的有机玻璃建造，这种材料能够承受的坦克和事故比玻璃更好的频繁流动。从底部水位15厘米，并应标明坦克，以确保水的体积是在小鼠一致。理想情况下，坦克的数量应该至少两次一次测试小鼠的数量很多，而第一套使用的是第二个水?…
Discussion
FST的（有时也被称为Porsolt游泳测试）是开发的第一对大鼠，小鼠Porsolt和同事 12,13，然后修改。除了 上述的协议，在我们的实验室中成功，主要是微妙的测试修改已公布（见Hascoët和Bourin 为一个完整的审查1）。它是一种常见的测试，使用的抗抑郁药物和各种行为和神经生物学基础和临床研究3,14-16操作的影响疗效评价。它被形容为渲染“行为绝望”是诱导情况，即动?…
Disclosures
The authors have nothing to disclose.
Acknowledgements
这项研究已被授予NIHM MH091816 R01和R21的MH084043到TDG支持。
Materials
- Water tanks
- Thermometer
- Timer
- Video Camera
- White Noise Generator (optional)
- Drying Paper
References
- Hascoét, M., Bourin, M. . In Mood and Anxiety Related Phenotypes in Mice. 42, 85-118 (2009).
- Sunal, R., Gümüçel, a. B., Kayaalp, S. O. Effect of changes in swimming area on results of “behavioral despair test”. Pharmacology Biochemistry and Behavior. 49, 891-896 (1994).
- Petit-Demouliere, B., Chenu, F., Bourin, M. Forced swimming test in mice: a review of antidepressant activity. Psychopharmacology. (Berl). 177, 245-255 (2005).
- Cryan, J. F., Markou, A., Lucki, I. Assessing antidepressant activity in rodents: recent developments and future needs. Trends in Pharmacological Sciences. 23, 238-245 (2002).
- Can, A. Antidepressant-like responses to lithium in genetically diverse mouse strains. Genes, Brain and Behavior. 10, 434-443 (2011).
- Lucki, I., Dalvi, A., Mayorga, A. J. Sensitivity to the effects of pharmacologically selective antidepressants in different strains of mice. Psychopharmacology. (Berl). 155, 315-322 (2001).
- David, D. J., Renard, C. E., Jolliet, P., Hascoet, M., Bourin, M. Antidepressant-like effects in various mice strains in the forced swimming test. Psychopharmacology (Berl). 166, 373-382 (2003).
- Bai, F., Li, X., Clay, M., Lindstrom, T., Skolnick, P. Intra- and interstrain differences in models of “behavioral despair”. Pharmacol. Biochem. Behav. 70, 187-192 (2001).
- Guzzetti, S. Strain differences in paroxetine-induced reduction of immobility time in the forced swimming test in mice: Role of serotonin. European Journal of Pharmacology. 594, 117-124 (2008).
- Cervo, L. Genotype-dependent activity of tryptophan hydroxylase-2 determines the response to citalopram in a mouse model of depression. J. Neurosci. 25, 8165-8172 (2005).
- Jiao, J., Nitzke, A., Doukas, D., Seiglie, M., Dulawa, S. Antidepressant response to chronic citalopram treatment in eight inbred mouse strains. Psychopharmacology. 213, 509-520 (2011).
- Porsolt, R. D., Pichon, M. L. e., Jalfre, M. Depression: a new animal model sensitive to antidepressant treatments. Nature. 266, 730-732 (1977).
- Porsolt, R. D., Bertin, A., Jalfre, M. Behavioral despair in mice: a primary screening test for antidepressants. Arch. Int. Pharmacodyn. Ther. 229, 327-336 (1977).
- Porsolt, R. D., Bertin, A., Jalfre, M. “Behavioural despair” in rats and mice: strain differences and the effects of imipramine. Eur. J. Pharmacol. 51, 291-294 (1978).
- Mineur, Y. S., Belzung, C., Crusio, W. E. Effects of unpredictable chronic mild stress on anxiety and depression-like behavior in mice. Behav. Brain. Res. 175, 43-50 (2006).
- Millstein, R. A., Holmes, A. Effects of repeated maternal separation on anxiety- and depression-related phenotypes in different mouse strains. Neuroscience & Biobehavioral Reviews. 31, 3-17 (2007).
- Cryan, J. F., Valentino, R. J., Lucki, I. Assessing substrates underlying the behavioral effects of antidepressants using the modified rat forced swimming test. Neuroscience & Biobehavioral Reviews. 29, 547-569 (2005).
- Crowley, J. J., Jones, O. ‘. L. e. a. r. y., F, O., Lucki, I. Automated tests for measuring the effects of antidepressants in mice. Pharmacology Biochemistry and Behavior. 78, 269-274 (2004).
- Kurtuncu, M., Luka, L. J., Dimitrijevic, N., Uz, T., Manev, H. Reliability assessment of an automated forced swim test device using two mouse strains. Journal of Neuroscience Methods. 149, 26-30 (2005).
- Hayashi, E., Shimamura, M., Kuratani, K., Kinoshita, M., Hara, H. Automated experimental system capturing three behavioral components during murine forced swim test. Life Sciences. 88, 411-417 (2011).
- Cryan, J., Page, M., Lucki, I. Differential behavioral effects of the antidepressants reboxetine, fluoxetine, and moclobemide in a modified forced swim test following chronic treatment. Psychopharmacology. 182, 335-344 (2005).
- Gould, T. D. Beta-catenin overexpression in the mouse brain phenocopies lithium-sensitive behaviors. Neuropsychopharmacology. 32, 2173-2183 (2007).
- Can, A., Grahame, N. J., Gould, T. D. Affect-related related behaviors in mice selectively bred for high and low voluntary alcohol consumption. Behav. Genet. , (2011).
- McKinney, W. T., Bunney, W. E. Animal Model of Depression: I Review of Evidence: Implications for Research.. Arch. Gen. Psychiatry. 21, 240-248 (1969).
- Willner, P. The validity of animal models of depression. Psychopharmacology.(Berl). 83, 1-16 (1984).
- Detke, M. J., Lucki, I. Detection of serotonergic and noradrenergic antidepressants in the rat forced swimming test: the effects of water depth. Behav. Brain Res. 73, 43-46 (1995).
- Gould, T. D., Dao, D. T., Kovacsics, C. E., Gould, T. D. . In Mood and Anxiety Related Phenotypes in mice: characterization using behavioral tests. 42, (2009).
- Bourin, M., Fiocco, A. J., Clenet, F. How valuable are animal models in defining antidepressant activity. Human Psychopharmacology: Clinical and Experimental. 16, 9-21 (2001).
Cite This Article
Can, A., Dao, D. T., Arad, M., Terrillion, C. E., Piantadosi, S. C., Gould, T. D. The Mouse Forced Swim Test. J. Vis. Exp. (59), e3638, doi:10.3791/3638 (2012).