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Protocols for Oral Infection of Lepidopteran Larvae with Baculovirus

Published: September 3, 2008 doi: 10.3791/888


In this video, we demonstrate oral infection techniques of lepidopteran larvae with baculovirus in order to determine insecticidal efficiency.


Baculoviruses are widely used both as protein expression vectors and as insect pest control agents. This video shows how lepidopteran larvae can be infected with polyhedra by droplet feeding and diet plug-based bioassays. This accompanying Springer Protocols section provides an overview of the baculovirus lifecycle and use of baculoviruses as insecticidal agents, including discussion of the pros and cons for use of baculoviruses as insecticides, and progress made in genetic enhancement of baculoviruses for improved insecticidal efficacy.


Please visit Springer Protocols to learn more about the engineering baculovirus as an insecidical agent and the oral infection techniques used in this assay.


The authors have nothing to disclose.


Oral Infection Lepidopteran Larvae Baculovirus Protein Expression Vectors Insect Pest Control Agents Droplet Feeding Diet Plug-based Bioassays Baculovirus Lifecycle Insecticidal Agents Genetic Enhancement Improved Insecticidal Efficacy
Protocols for Oral Infection of Lepidopteran Larvae with Baculovirus
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Cite this Article

Sparks, W., Li, H., Bonning, B.More

Sparks, W., Li, H., Bonning, B. Protocols for Oral Infection of Lepidopteran Larvae with Baculovirus. J. Vis. Exp. (19), e888, doi:10.3791/888 (2008).

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