Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove

Biology

Protocols for Oral Infection of Lepidopteran Larvae with Baculovirus

doi: 10.3791/888 Published: September 3, 2008

Summary

In this video, we demonstrate oral infection techniques of lepidopteran larvae with baculovirus in order to determine insecticidal efficiency.

Abstract

Baculoviruses are widely used both as protein expression vectors and as insect pest control agents. This video shows how lepidopteran larvae can be infected with polyhedra by droplet feeding and diet plug-based bioassays. This accompanying Springer Protocols section provides an overview of the baculovirus lifecycle and use of baculoviruses as insecticidal agents, including discussion of the pros and cons for use of baculoviruses as insecticides, and progress made in genetic enhancement of baculoviruses for improved insecticidal efficacy.

Protocol

Please visit Springer Protocols to learn more about the engineering baculovirus as an insecidical agent and the oral infection techniques used in this assay.

Disclosures

The authors have nothing to disclose.

Protocols for Oral Infection of Lepidopteran Larvae with Baculovirus
Play Video
PDF DOI

Cite this Article

Sparks, W., Li, H., Bonning, B. Protocols for Oral Infection of Lepidopteran Larvae with Baculovirus. J. Vis. Exp. (19), e888, doi:10.3791/888 (2008).More

Sparks, W., Li, H., Bonning, B. Protocols for Oral Infection of Lepidopteran Larvae with Baculovirus. J. Vis. Exp. (19), e888, doi:10.3791/888 (2008).

Less
Copy Citation Download Citation Reprints and Permissions
View Video

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
simple hit counter