Induced Ovulation
Induced Ovulation - Related Videos
05:41
In Vitro Ovule Cultivation for Live-cell Imaging of Zygote Polarization and Embryo Patterning in Arabidopsis thaliana
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Cited by 27 •
2017
This manuscript describes an in vitro ovule cultivation method that enables live-cell imaging of Arabidopsis zygotes and embryos. This method is utilized to visualize the intracellular dynamics during zygote polarization and the cell fate specification in developing embryos.
03:20
Microinjection of Tetrodotoxin into the Brain of an Awake Female Rat to Inhibit Ovulation
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2025
Source: Silva, C. et al. Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections. J. Vis. Exp. (2020)This video demonstrates the protocol for microinjection of tetrodotoxin (TTX) into the hypothalamus of a freely moving rat to transiently inhibit neuronal activity, suppress gonadotropin-releasing hormone secretion, and prevent ovulation.
04:02
Embryo Transfer Into Rabbit: A Procedure for Laparoscopy-Guided Transfer of Embryos Into Oviduct of Ovulating Recipient Female Rabbit Model
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2025
This video describes the technique of transferring embryos into the oviduct of a recipient female rabbit via Laparoscopy. It allows embryo remodeling in rabbits during its migration through the oviduct, eventually helping in successful implantation. This technique can also be used for stored embryos with good efficiency.
11:04
Unraveling the Role of Discrete Areas of the Rat Brain in the Regulation of Ovulation through Reversible Inactivation by Tetrodotoxin Microinjections
0 Views •
2020
This protocol describes the construction of a low-cost microinjection system, its stereotaxic implantation into deep-brain structures and the procedure for timed microinjections of tetrodotoxin in awake and unrestrained rats. The goal is to reveal the participation of hypothalamic structures in the regulation of ovulation by inhibiting their neural activity.
09:33
An Efficient Method for Quantitative, Single-cell Analysis of Chromatin Modification and Nuclear Architecture in Whole-mount Ovules in Arabidopsis
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Cited by 15 •
2014
We provide here an efficient and reliable protocol for immunostaining, Fluorescence in situ Hybridization, DNA staining followed by quantitative, high-resolution imaging in whole-mount Arabidopsis thaliana ovules. This method was successfully used to analyze chromatin modifications and nuclear architecture.
FAQs
Successful induction depends on coordinating two linked events: maturation of the ovarian follicle and an ovulatory luteinizing hormone surge. The trigger is therefore not simply egg release in isolation; it provokes the endocrine sequence that precedes it. This relationship makes induced ovulation useful for investigating how ovarian function connects hormonal signaling with later reproductive events.
Physiological induction relies on a reproductive stimulus that provokes the body's ovulatory sequence, whereas medical induction uses an intervention to provide that stimulus or trigger. Both approaches are studied through the same biological endpoint, but they differ in how the initiating signal is supplied. This distinction helps separate normal reproductive regulation from experimentally or clinically managed control.
The luteinizing hormone surge matters because it marks the hormonal transition associated with egg release after follicle maturation. Induced ovulation aims to provoke this surge, directly or through an intervention, as part of the ovulatory sequence. Studying that relationship lets biologists examine connections among endocrine signaling, ovarian activity, and the timing of reproductive processes.
Depending on the setting, gonadotropins, human chorionic gonadotropin, or gonadotropin-releasing hormone may be used to provoke the hormonal events associated with ovulation. The available source identifies these as intervention options rather than a single universal protocol. Their use reflects the need to initiate the relevant endocrine trigger in clinical or research applications.
In fertility treatment, induced ovulation can help manage reproduction by coordinating a hormonal trigger with a reproductive goal. In animals, the same principle supports timed breeding, allowing reproductive events to be organized around a deliberate schedule. These applications show how controlling ovulation timing connects ovarian physiology with fertilization and broader reproductive success.
In biology research, induced ovulation provides a way to examine ovarian function, fertilization, and factors that influence reproductive success. Because the egg-release trigger is deliberately initiated, investigators can focus on how follicle maturation and hormonal signaling relate to later reproductive outcomes. The approach therefore links ovarian events with broader questions about reproduction.