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JoVE Journal
Developmental Biology

This content is Open Access.

Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart
 

Analysis of Cardiomyocyte Development using Immunofluorescence in Embryonic Mouse Heart

Article doi: 10.3791/52644
March 26th, 2015 Usage Statistics

Summary March 26th, 2015

Mutations that lead to congenital heart defects benefit from in vivo investigation of cardiac structure during development, but high-resolution structural studies in the mouse embryonic heart are technically challenging. Here we present a robust immunofluorescence and image analysis method to assess cardiomyocyte-specific structures in the developing mouse heart.

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