Neonatal Microglia
Neonatal Microglia - Related Videos
11:28
Culturing Microglia from the Neonatal and Adult Central Nervous System
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Cited by 35 •
2013
We outline methods for the efficient and quick isolation/culture of viable microglia from the neonatal cerebral cortex and adult spinal cord. The dissection and plating of cortical microglia can be accomplished within 90 minutes, with the subsequent microglial harvest taking place ~ 10 days following the initial dissection.
09:12
Isolation of Cortical Microglia with Preserved Immunophenotype and Functionality From Murine Neonates
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Cited by 17 •
2014
One key to successful investigation of microglial biology is the preservation of microglial immunofunction ex vivo during isolation from CNS tissue. Isolating microglia via rotary shaking results in highly pure and immunofunctional cell cultures as assessed by fluorescent imaging, immunocytochemistry, and ELISA following microglia activation with the proinflammatory stimuli lipopolysaccharide (LPS) and Pam3CSK4 (Pam).
10:20
Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue
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Cited by 133 •
2012
Isolating primary microglia from the cellular heterogeneity of the brain is essential to investigate their role in both physiological and pathological conditions. This protocol describes a mechanical isolation and mixed cell culture technique that provides high yield and high purity, viable primary microglial cells for in vitro study and downstream applications.
02:26
Obtaining a Mixed Glial Cell Culture from a Neonatal Mouse Brain
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2025
The video demonstrated the method of isolating and culturing cells from a neonatal mouse brain. It involved enzymatic and mechanical dissociation of the brain, followed by culturing in growth media lacking neuronal growth factor to obtain a mixed glial population.
07:54
Isolation of Neonatal Extrahepatic Cholangiocytes
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Cited by 17 •
2014
A technique to isolate cholangiocytes from the extrahepatic bile ducts of neonatal mice is described. The ducts are meticulously dissected, and then cells are isolated by outgrowth in thick collagen gels. This method provides a useful tool for studying extrahepatic bile duct development and pathology.
FAQs
Neonatal microglia detect pathogen-associated molecular patterns and damage-associated molecular patterns through receptors such as Toll-like receptors. These signals can change their cellular state and promote cytokine release, engulfment of material, or synaptic pruning. Studying these responses helps clarify how infection and sterile tissue damage may produce overlapping or distinct effects during early central nervous system development.
Synaptic pruning allows neonatal microglia to remove selected developing synapses while neural circuits are being organized. Because inflammatory signals can alter microglial state, infection or tissue damage may influence which neural connections are engulfed. This links immune sensing with circuit development and provides a mechanism for investigating how early-life inflammation could contribute to later neurological outcomes.
Their immune activities can be protective and potentially disruptive at the same time. Cytokine release and engulfment support responses to infection or damage, whereas changes in these activities during circuit formation may affect neural organization. This balance is central to immunology and infection research because effective defense must be considered alongside the possibility of harmful neuroinflammation in the developing brain.
Researchers can examine changes in microglial state, cytokine release, engulfment of material, and synaptic pruning. Together, these readouts connect receptor-mediated sensing with functional effects on developing neural circuits. Comparing such responses after pathogen-associated or damage-associated stimulation can help investigators relate early immune activity to host defense, neurodevelopment, and possible long-term neurological consequences.
They provide a cellular framework for examining how exposure to pathogens or inflammation affects the developing central nervous system. Investigators can relate immune sensing and microglial responses to changes in neural circuit organization, host defense, and later neurological outcomes. This approach is especially relevant when studying why the developing brain may be vulnerable to infection-associated inflammatory effects.
Their responses identify the connection between immune detection and potentially harmful neuroinflammation during development. Understanding when cytokine release, engulfment, or synaptic pruning becomes damaging can inform investigations of approaches intended to limit excessive inflammatory effects without disregarding host defense. The same framework also supports research into how early pathogen exposure may shape neurological outcomes over time.