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Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids
JoVE Journal
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Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids

Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids

12,850 Views

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10:44 min

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December 28, 2015

DOI:

10.3791/53486-v

DOI:
10.3791/53486-v

•

10:44 min
December 28, 2015

•

11 Views
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1The Centenary Institute, 2Sydney Medical School,University of Sydney, 3The University of Queensland Diamantina Institute, Translational Research Institute,The University of Queensland, 4Dermatoloji Anabilim Dalı,Royal Prince Alfred Hospital, 5Discipline of Dermatology,University of Sydney

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We describe two complementary methods using the fluorescence ubiquitination cell cycle indicator (FUCCI) and image analysis or flow cytometry to identify and isolate cells in the inner G1 arrested and outer proliferating regions of 3D spheroids.

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Beaumont, K. A., Anfosso, A., Ahmed, F., Weninger, W., Haass, N. K. Imaging- and Flow Cytometry-based Analysis of Cell Position and the Cell Cycle in 3D Melanoma Spheroids. J. Vis. Exp. (106), e53486, doi:10.3791/53486 (2015).

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