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JoVE Journal
Biology

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Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy
 

Simultaneous Multicolor Imaging of Biological Structures with Fluorescence Photoactivation Localization Microscopy

Article DOI: 10.3791/50680-v 12:51 min December 9th, 2013
December 9th, 2013

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Summary

We demonstrate the use of fluorescence photo activation localization microscopy (FPALM) to simultaneously image multiple types of fluorescently labeled molecules within cells. The techniques described yield the localization of thousands to hundreds of thousands of individual fluorescent labeled proteins, with a precision of tens of nanometers within single cells.

Tags

Super Resolution Microscopy Fluorescence Photoactivation Localization Microscopy Single Molecule Imaging Spatial Resolution Photoactivatable Fluorescent Proteins Photoswitchable Fluorescent Proteins Organic Dyes Antibodies Nanoscale Spatial Distributions Diffraction Limit Cellular Organization Fluorescence Microscopy
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