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Assessing Hepatic Steatosis with 3D Fluorescence Lipid Droplet Reconstruction

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Image Acquisition of Lipid Droplets and Analysis (Video) | JoVE

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Image Acquisition of Lipid Droplets and Analysis

Image Acquisition of Lipid Droplets and Analysis (Video) | JoVE

Assessing Hepatic Steatosis with 3D Fluorescence Lipid Droplet Reconstruction

DOI:

10.3791/200053-v

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04:50 min

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June 02, 2023

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Karina Garcia^2,3,4, André Alves^2,3, Teresa M. Ribeiro-Rodrigues^2,3, Flávio Reis^2,3, Sofia Viana^2,3,4

¹Institute of Pharmacology & Experimental Therapeutics & Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine,University of Coimbra, ²Center for Innovative Biomedicine and Biotechnology (CIBB),University of Coimbra, ³Clinical Academic Center of Coimbra (CACC), ⁴Polytechnic Institute of Coimbra,Coimbra Health School

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Keywords: Lipid Droplets Image Acquisition Confocal Microscopy BODIPY 493/503 DAPI Laser Scanning Sequential Scan 20X Objective 10X Objective 40X Objective Z-stack Orthogonal Views 3D Imaging CellProfiler Image Processing Identify Primary Objects Measure Object Intensity Filter Objects

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