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Assessing Hepatic Steatosis with 3D Fluorescence Lipid Droplet Reconstruction

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Frozen Liver Tissue Sectioning and BODIPY Staining of Lipid Droplets (Video) | JoVE

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Frozen Liver Tissue Sectioning and BODIPY Staining of Lipid Droplets

Frozen Liver Tissue Sectioning and BODIPY Staining of Lipid Droplets (Video) | JoVE

Assessing Hepatic Steatosis with 3D Fluorescence Lipid Droplet Reconstruction

DOI:

10.3791/200052-v

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01:18 min

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June 02, 2023

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Karina Garcia^2,3,4, André Alves^2,3, Teresa M. Ribeiro-Rodrigues^2,3, Flávio Reis^2,3, Sofia Viana^2,3,4

¹Institute of Pharmacology & Experimental Therapeutics & Coimbra Institute for Clinical and Biomedical Research (iCBR), Faculty of Medicine,University of Coimbra, ²Center for Innovative Biomedicine and Biotechnology (CIBB),University of Coimbra, ³Clinical Academic Center of Coimbra (CACC), ⁴Polytechnic Institute of Coimbra,Coimbra Health School

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Cryostat Sectioning BODIPY Staining Lipid Droplets PBS Wash BODIPY 493/503 DAPI Fluorescence Microscopy Coverslip Mounting

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