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Functional Site-Directed Fluorometry in Native Skeletal Muscle Cells

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Construct Design for Transfecting Cysteine-Engineered Voltage-Gated Ca2+ Channels in Murine Isolated Skeletal Muscle Fibers (Video) | JoVE

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JoVE Journal Biology

Construct Design for Transfecting Cysteine-Engineered Voltage-Gated Ca²⁺ Channels in Murine Isolated Skeletal Muscle Fibers

Construct Design for Transfecting Cysteine-Engineered Voltage-Gated Ca2+ Channels in Murine Isolated Skeletal Muscle Fibers (Video) | JoVE

Functional Site-Directed Fluorometry in Native Skeletal Muscle Cells

DOI:

10.3791/200065-v

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01:22 min

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June 02, 2023

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Hugo Bibollet, Daniel F. Bennett, Martin F. Schneider, Erick O. Hernández-Ochoa

¹Department of Biochemistry and Molecular Biology,University of Maryland School of Medicine

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Keywords: Cysteine-engineered Voltage-gated Ca2+ Channels Murine Isolated Skeletal Muscle Fibers CaV1.1 CDNA Construct EGFP-tagged Alpha Subunit Cytomegalovirus Promoter Site-directed Mutagenesis S4 Membrane Extracellular Interface GFP Observation Ionic Current Recording

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