/

/

Functional Site-Directed Fluorometry in Native Skeletal Muscle Cells

/

Constructontwerp voor het transfecteren van cysteïne-engineered voltage-gated Ca^{2 +} kanalen in murine geïsoleerde skeletspiervezels

JoVE Journal
Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biology

Construct Design for Transfecting Cysteine-Engineered Voltage-Gated Ca²⁺ Channels in Murine Isolated Skeletal Muscle Fibers

Please note that all translations are automatically generated. Click here for the English version.

Constructontwerp voor het transfecteren van cysteïne-engineered voltage-gated Ca^{2 +} kanalen in murine geïsoleerde skeletspiervezels

Functional Site-Directed Fluorometry in Native Skeletal Muscle Cells

DOI:

10.3791/200065-v

•

01:22 min

•

June 02, 2023

•

Hugo Bibollet, Daniel F. Bennett, Martin F. Schneider, Erick O. Hernández-Ochoa

¹Department of Biochemistry and Molecular Biology,University of Maryland School of Medicine

Tags

Keywords: Cysteine-engineered Voltage-gated Ca2+ Channels Murine Isolated Skeletal Muscle Fibers CaV1.1 CDNA Construct EGFP-tagged Alpha Subunit Cytomegalovirus Promoter Site-directed Mutagenesis S4 Membrane Extracellular Interface GFP Observation Ionic Current Recording

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Functionele site-directed fluorometrie in inheemse cellen om de prikkelbaarheid van skeletspieren te bestuderen

Constructontwerp voor het transfecteren van cysteïne-engineered voltage-gated Ca^{2 +} kanalen in murine geïsoleerde skeletspiervezels

Beoordeling van EGFP-positieve vezel elektrische activiteit, cysteïnekleuring en signaalacquisitie

Read Article