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Journal
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Biology
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Functional Site-Directed Fluorometry in Native Skeletal Muscle Cells
/
システイン工学的電位依存性
Ca2+
チャネルをマウス単離骨格筋線維にトランスフェクトするための設計の構築(英語)
JoVE Journal
Biology
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JoVE Journal
Biology
Construct Design for Transfecting Cysteine-Engineered Voltage-Gated Ca
2+
Channels in Murine Isolated Skeletal Muscle Fibers
Please note that all translations are automatically generated.
Click here for the English version.
システイン工学的電位依存性
Ca2+
チャネルをマウス単離骨格筋線維にトランスフェクトするための設計の構築(英語)
Functional Site-Directed Fluorometry in Native Skeletal Muscle Cells
DOI:
10.3791/200065-v
•
01:22 min
•
June 02, 2023
•
Hugo Bibollet
,
Daniel F. Bennett
,
Martin F. Schneider
,
Erick O. Hernández-Ochoa
1
Department of Biochemistry and Molecular Biology
,
University of Maryland School of Medicine
Tags
Keywords: Cysteine-engineered Voltage-gated Ca2+ Channels
Murine Isolated Skeletal Muscle Fibers
CaV1.1 CDNA Construct
EGFP-tagged Alpha Subunit
Cytomegalovirus Promoter
Site-directed Mutagenesis
S4 Membrane Extracellular Interface
GFP Observation
Ionic Current Recording
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