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Functional Site-Directed Fluorometry in Native Skeletal Muscle Cells
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Konstruer design for transfeksjon av cystein-konstruerte spenningsstyrte Ca2+ kanaler i Murine isolerte skjelettmuskelfibre
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JoVE Journal Biology
Construct Design for Transfecting Cysteine-Engineered Voltage-Gated Ca2+ Channels in Murine Isolated Skeletal Muscle Fibers

Konstruer design for transfeksjon av cystein-konstruerte spenningsstyrte Ca2+ kanaler i Murine isolerte skjelettmuskelfibre

Functional Site-Directed Fluorometry in Native Skeletal Muscle Cells

DOI:
10.3791/200065-v

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01:22 min

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June 02, 2023

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, , ,
1Department of Biochemistry and Molecular Biology,University of Maryland School of Medicine

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Keywords: Cysteine-engineered Voltage-gated Ca2+ ChannelsMurine Isolated Skeletal Muscle FibersCaV1.1 CDNA ConstructEGFP-tagged Alpha SubunitCytomegalovirus PromoterSite-directed MutagenesisS4 Membrane Extracellular InterfaceGFP ObservationIonic Current Recording

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