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Developmental Biology

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Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing

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Obtaining Wildtype and Transgenic Zebrafish Larvae and WildType Whole Larvae Dissociation (Video) | JoVE

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Developmental Biology

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JoVE Journal Developmental Biology

Obtaining Wildtype and Transgenic Zebrafish Larvae and WildType Whole Larvae Dissociation

Obtaining Wildtype and Transgenic Zebrafish Larvae and WildType Whole Larvae Dissociation (Video) | JoVE

Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing

DOI:

10.3791/200859-v

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01:30 min

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November 10, 2023

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Naomi J. M. Kakiailatu, Laura E. Kuil², Eric Bindels, Joke T. M. Zink, Michael Vermeulen, Veerle Melotte, Maria M. Alves

¹Department of Clinical Genetics,Erasmus University Medical Center – Sophia Children’s Hospital, ²Division of Psychosocial Research and Epidemiology,the Netherlands Cancer Institute, ³Department of Hematology,Erasmus Medical Center, ⁴Department of Pathology, GROW-School for Oncology and Developmental Biology,Maastricht University Medical Center

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Keywords: Zebrafish Wildtype Transgenic Larvae Dissociation HEPES-buffered E3 Medium Trypsin-EDTA Anesthesia Tricaine

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