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Developmental Biology

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Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing

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Isolation and Dissociation of Gut from Zebrafish Larvae for FACS Enrichment of Gut Cells (Video) | JoVE

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Developmental Biology

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JoVE Journal Developmental Biology

Isolation and Dissociation of Gut from Zebrafish Larvae for FACS Enrichment of Gut Cells

Isolation and Dissociation of Gut from Zebrafish Larvae for FACS Enrichment of Gut Cells (Video) | JoVE

Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing

DOI:

10.3791/200860-v

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02:37 min

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November 10, 2023

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Naomi J. M. Kakiailatu, Laura E. Kuil², Eric Bindels, Joke T. M. Zink, Michael Vermeulen, Veerle Melotte, Maria M. Alves

¹Department of Clinical Genetics,Erasmus University Medical Center – Sophia Children’s Hospital, ²Division of Psychosocial Research and Epidemiology,the Netherlands Cancer Institute, ³Department of Hematology,Erasmus Medical Center, ⁴Department of Pathology, GROW-School for Oncology and Developmental Biology,Maastricht University Medical Center

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Keywords: Gut Zebrafish Larvae FACS Cell Isolation Dissociation Papain Agarose Plate HEPES E3 Medium Dissection Intestine Cell Strainer Centrifugation DAPI Cell Labeling

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