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Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies
JoVE Journal
Biochemistry
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JoVE Journal Biochemistry
Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

Sample Preparation using a Lipid Monolayer Method for Electron Crystallographic Studies

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04:22 min

November 20, 2021

DOI:

04:22 min
November 20, 2021

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Summary

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Lipid monolayers have been used as a foundation for forming two-dimensional (2D) protein crystals for structural studies for decades. They are stable at the air-water interface and can serve as a thin supporting material for electron imaging. Here we present the proven steps on preparing lipid monolayers for biological studies.

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