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Chapters
- 00:05Title
- 01:44Pretreatment of Cells and Metaphase Preparation
- 05:16Pepsin Pretreatment of Slides
- 07:32Chromosome and Probe Denaturation and Hybridization
- 09:00Fluorescent Probe Design
- 10:53Representative Results from Spectral Karyotyping of Mouse and Human Chromosomes
- 12:31Conclusion
Spectral Karyotyping (SKY) is an advanced cytogenetics technique to identify genomic and chromosomal aberrations. This technique takes advantage of chromosome painting probes, which allow classification of all chromosomes. SKY can also identify complex chromosome aberrations and segregation defects in mice and humans with various diseases, including polycystic kidney disease.
Tags
Spectral KaryotypingChromosome AbnormalitiesHumansMicePolycystic Kidney DiseaseBanding PatternClassical Banding TechniqueReliable IdentificationComplex Chromosomal AberrationsCancerSpectral Karyotyping (SKY)Multicolor Fluorescence In-situ Hybridization (FISH)Spectral MicroscopeCytogenetic AnalysisGenetic DiseasesMalignanciesMulticolor Fluorescently-labelled DNA ProbesDegenerate Oligonucleotide PrimersPCRUnique Spectral ColorIn-situ HybridizationFluorescent DyesRhodamineTexas RedCy5FITCCy5.5Chromosome Specific Probes
