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Chapters
- 00:05Title
- 01:09Preparation of Instruments and Anesthetic
- 02:21Zebrafish Husbandry and Pre-injection Treatment
- 03:33Microinjection
- 05:27Imaging Acquisition and Processing
- 07:08Results: Injection of Rhodamine Dextran in bbs9 Morpholino Knockdown Zebrafish
- 07:37Conclusion
The zebrafish is a popular tool to model chronic kidney disease (CKD). However, their small size makes it impossible to evaluate renal function using traditional methods. We describe a fluorescent dye kidney clearance assay1 that allows quantitative analysis of zebrafish kidney function in CKD.
