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Evaluation of Zebrafish Kidney Function Using a Fluorescent Clearance Assay
JoVE Journal
Biology
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JoVE Journal Biology
Evaluation of Zebrafish Kidney Function Using a Fluorescent Clearance Assay
DOI:

08:13 min

February 20, 2015

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Chapters

  • 00:05Title
  • 01:09Preparation of Instruments and Anesthetic
  • 02:21Zebrafish Husbandry and Pre-injection Treatment
  • 03:33Microinjection
  • 05:27Imaging Acquisition and Processing
  • 07:08Results: Injection of Rhodamine Dextran in bbs9 Morpholino Knockdown Zebrafish
  • 07:37Conclusion

Summary

Automatic Translation

The zebrafish is a popular tool to model chronic kidney disease (CKD). However, their small size makes it impossible to evaluate renal function using traditional methods. We describe a fluorescent dye kidney clearance assay1 that allows quantitative analysis of zebrafish kidney function in CKD.

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