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JoVE Journal
Immunology and Infection

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Preparation of Viral DNA from Nucleocapsids

Preparation of Viral DNA from Nucleocapsids

Article DOI: 10.3791/3151
August 16th, 2011


Summary August 16th, 2011

We describe the process of isolating high purity herpesvirus nucleocapsid DNA from infected cells. The final DNA captured from solution is of high concentration and purity, making it ideally suited for high-throughput sequencing, high fidelity PCR reactions, and transfections to produce new viral recombinants.

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