Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

The overall goal of this method is to purify Herpes Simplex virus type one DNA from infected cells for the proteomic analysis of viral genome associated proteins. This method provides key insights into the involvement of cellular factors and Herpes virus infection. The main advantage of this technique is that it can be adapted to directly purify different populations of viral DNA from infected cells, allowing for the analysis of multiple aspects of infection.

Begin this procedure with culturing of MRC five cells as described in the text protocol. Then, dilute HSV-1 in cold tris-buffered saline or TBS. For the following steps, it is important to turn off the blower and the tissue culture hood to prevent cells from drying out.