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Immunology and Infection
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Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins
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Immunology and Infection
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JoVE Journal Immunology and Infection
Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

Purification of Viral DNA for the Identification of Associated Viral and Cellular Proteins

DOI:
10.3791/56374-v

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08:26 min

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August 31, 2017

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,
1Department of Microbiology and Molecular Genetics,University of Pittsburgh School of Medicine

Chapters

  • 00:05Title
  • 00:37Viral Infection and EdC Labeling
  • 01:57Purification of Viral DNA and Associated Proteins
  • 06:21Results: Protein Purification and Proteomics Data
  • 07:36Conclusion

Summary

Automatic Translation

Automatic Translation

The goal of this protocol is to specifically tag and selectively isolate viral DNA from infected cells for the characterization of viral genome associated proteins.

Tags

Keywords: Viral DNA PurificationHerpes Simplex Virus Type 1Proteomic AnalysisCellular FactorsViral GenomeMRC-5 CellsHSV-1 InfectionViral DNA ReplicationEdC LabelingNuclei ExtractionNuclear PelletClick ReactionCellular Proteins

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