A Quantitative Evaluation of Cell Migration by the Phagokinetic Track Motility Assay

The overall goal of this procedure is to be able to quantitatively measure cellular motility over time using a gold nanoparticle based cell motility assay. This is accomplished by first preparing glass cover slips for a coating of gold nanoparticles. Specifically endotoxin free cover slips are coated with gelatin and then baked.

The second step is to reduce chloro oric acid, which will create gold nanoparticles and to coat the gelatin coated cover slips evenly with the solution. Next, the cells are placed onto the gold nanoparticle coating cover slips for the desired timeframe of the experiment cells. Moving on, the gelatin will displace gold nanoparticles creating tracks.