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A Quantitative Evaluation of Cell Migration by the Phagokinetic Track Motility Assay
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1Department of Microbiology and Immunology,Louisiana State University Health Sciences Center, 2Center for Molecular and Tumor Virology,Louisiana State University Health Sciences Center, 3Department of Microbiology and Immunology,SUNY Upstate Medical University, 4Feist-Weiller Cancer Center,Louisiana State University Health Sciences Center
Chapters
- 00:05Title
- 02:20Preparation of Gelatin-coated Coverslips
- 04:00Preparation of Colloidal Gold-coated Coverslips
- 07:41Analysis of Cellular Motility
- 09:40Results: Cell Motility Paths in Colloidal Gold Particles
- 10:33Conclusion
The phagokinetic motility track assay is a method used to assess the movement of cells. Specifically, the assay measures chemokinesis (random cell motility) over time in a quantitative manner. The assay takes advantage of the ability of cells to create a measurable track of their movement on colloidal gold-coated coverslips.
Tags
Cell MigrationPhagokinetic Track Motility AssayQuantitative EvaluationCellular MotilityUnicellular OrganismsMulticellular OrganismsImmune SurveillanceWound HealingNeurological DiseasesCardiovascular DiseasesImmunological DiseasesTumor FormationActin PolymerizationReceptor RecyclingCellular ExtensionsLamellipodiaCell MotilityMicroscopyQuantitative MeasurementResource-consuming Process
