Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

Andreia Gianotti Sommer; Sarah S. Rozelle; Spencer Sullivan; Jason A. Mills; Seon-Mi Park; Brenden W. Smith; Amulya M. Iyer; Deborah L. French; Darrell N. Kotton; Paul Gadue; George J. Murphy; Gustavo Mostoslavsky

doi:10.3791/4327

Journal

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Biology

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Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

JoVE Journal
Biology

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JoVE Journal Biology

Generation of Human Induced Pluripotent Stem Cells from Peripheral Blood Using the STEMCCA Lentiviral Vector

26,392 Views

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12:03 min

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October 31, 2012

DOI:

10.3791/4327-v

DOI:

10.3791/4327-v

•

12:03 min

October 31, 2012

•

5 Views

Andreia Gianotti Sommer¹, Sarah S. Rozelle¹, Spencer Sullivan², Jason A. Mills³, Seon-Mi Park¹, Brenden W. Smith¹, Amulya M. Iyer¹, Deborah L. French³, Darrell N. Kotton¹, Paul Gadue³, George J. Murphy¹, Gustavo Mostoslavsky¹

¹Center for Regenerative Medicine (CReM),Boston University School of Medicine, ²Department of Hematology,Children’s Hospital of Philadelphia, ³Center for Cellular and Molecular Therapeutics,Children’s Hospital of Philadelphia

Summary

Here we show a simple and effective protocol for the generation of human iPSCs from 3-4 ml of peripheral blood using a single lentiviral reprogramming vector. Reprogramming of readily available blood cells promises to accelerate the utilization of iPSC technology by making it accessible to a broader research community.