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Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells
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Developmental Biology
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JoVE Journal Developmental Biology
Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

Direct Induction of Hemogenic Endothelium and Blood by Overexpression of Transcription Factors in Human Pluripotent Stem Cells

DOI:
10.3791/52910-v

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08:14 min

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December 03, 2015

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, ,
1Primate Research Center,University of Wisconsin-Madison, 2Department of Pathology and Laboratory Medicine,University of Wisconsin School of Medicine and Public Health

Chapters

  • 00:05Title
  • 00:58Induction of Hematoendothelial Precursors from Human Pluripotent Stem Cells
  • 03:29Induction of Hematoendothelial Precursors from hPSCs (Day 1-7)
  • 05:29Results: Differentiation of Blood Cells from Endothelial Cells
  • 06:35Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the efficient induction of hemogenic endothelium and multipotential hematopoietic progenitors from human pluripotent stem cells via the forced expression of transcription factors.

Tags

Hemogenic EndotheliumHematopoietic CellsPluripotent Stem CellsTranscription FactorsETV2GATA1GATA2TAL1LMO2Endothelial-hematopoietic TransitionErythroidMegakaryocyticIn Vitro Modeling

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