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Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors
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Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors

Efficient iPS Cell Generation from Blood Using Episomes and HDAC Inhibitors

DOI:
10.3791/52009-v

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08:14 min

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October 28, 2014

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, , , , ,
1Clinical Research Division,Fred Hutchinson Cancer Research Center, 2Division of Hematology,The Children’s Hospital of Philadelphia, 3Department of Pathology,The Children’s Hospital of Philadelphia

Chapters

  • 00:05Title
  • 01:23Ficoll Separation of PBMCs and Expansion of Erythroblasts Days 0-6
  • 03:17Reprogramming Cells by Nulceofection Day 9
  • 04:51Plating Feeder Cells Day 10-11
  • 05:47Plating Cells on Feeder Cells and Changing Medium Day 12
  • 06:41Results: Representative Nucleofected and Inducible Pluripotent Stem (iPS) Cells
  • 07:46Conclusion

Summary

Automatic Translation

Automatic Translation

Here we describe a protocol for generating human induced pluripotent stem cells from peripheral blood using an episome based reprogramming strategy and histone deacetylase inhibitors.

Tags

Keywords: IPS Cell GenerationEpisomesHDAC InhibitorsPeripheral BloodIntegration-freeNonintegrating Reprogramming VectorsErythroblastsNucleofectionEBNA1Mouse Embryonic FibroblastsEpigenetic Remodeling

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