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Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans
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Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans

Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans

English

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12,202 Views

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11:22 min

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February 13, 2013

DOI:

10.3791/4373-v

DOI:
10.3791/4373-v

•

11:22 min
February 13, 2013

•

2 Views
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1Department of Science,Borough of Manhattan Community College, City Universtiy of New York (CUNY), 2Department of Biology,Queens College, The City University of New York (CUNY), 3Biochemistry Program, The Graduate Center,Queens College, The City University of New York (CUNY)

Summary

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We demonstrate how to use the RNAi feeding technique to knock down target genes and score body size phenotype in C. elegans. This method could be used for a large scale screen to identify potential genetic components of interest, such as those involved in body size regulation by DBL-1/TGF-β signaling.

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Liang, J., Xiong, S., Savage-Dunn, C. Using RNA-mediated Interference Feeding Strategy to Screen for Genes Involved in Body Size Regulation in the Nematode C. elegans. J. Vis. Exp. (72), e4373, doi:10.3791/4373 (2013).

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