Journal
/
Biology
/
Osmotic Avoidance in Caenorhabditis elegans: Synaptic Function of Two Genes, Orthologues of Human NRXN1 and NLGN1, as Candidates for Autism
JoVE Journal
Biology
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Osmotic Avoidance in Caenorhabditis elegans: Synaptic Function of Two Genes, Orthologues of Human NRXN1 and NLGN1, as Candidates for Autism

Osmotic Avoidance in Caenorhabditis elegans: Synaptic Function of Two Genes, Orthologues of Human NRXN1 and NLGN1, as Candidates for Autism

DOI:
10.3791/1616-v

•

11:20 min

•

December 11, 2009

•

, ,
1Departamento de Genética,Facultad de Ciencias, Universidad de Córdoba, 2Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC)

Chapters

  • 00:00Title
  • 00:31Introduction
  • 01:41Fructose Ring Assay
  • 04:24Behavioral Analysis of Knockout Animals
  • 05:33RNAi Feeding
  • 07:23Worm Handling and Scoring
  • 08:24Behavior Analysis of Knockdown Animals
  • 09:30Summary

Summary

Automatic Translation

Automatic Translation

Neurexins and neuroligins are membrane-neuron adhesion proteins which perform essential roles in synaptic differentiation and transmission. Neuroligin deficient mutants of C. elegans are defective in detecting osmotic strength, but when they also contain a mutation in the gene coding neurexin, they recover the wild type phenotype.

Tags

Osmotic AvoidanceCaenorhabditis ElegansSynaptic FunctionGenesNRXN1NLGN1AutismNeurexinsNeuroliginsExcitatory SynapsesInhibitory SynapsesPresynaptic MembranesPostsynaptic MembranesNeuronal ConnectionsDevelopment Of Human Nervous SystemAutism Spectrum DisordersExperimental ToolLaboratory ExperimentationNrx-1 GeneNlg-1 GeneAlpha-neurexin-1 ProteinNeuroligin-1 ProteinFunctional DomainsAmphid Sensory Organ

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/1616?article_type=v

Isolation and In vitro Activation of Caenorhabditis elegans Sperm
check_url/1616?article_type=v

Time-lapse Microscopy of Early Embryogenesis in Caenorhabditis elegans
check_url/1616?article_type=v

Basic Caenorhabditis elegans Methods: Synchronization and Observation
check_url/1616?article_type=v

Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans
check_url/1616?article_type=v

Prostaglandin Extraction and Analysis in Caenorhabditis elegans
check_url/1616?article_type=v

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
check_url/1616?article_type=v

Dietary Supplementation of Polyunsaturated Fatty Acids in Caenorhabditis elegans
check_url/1616?article_type=v

Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy
check_url/1616?article_type=v

Measurement of Oxygen Consumption Rates in Intact Caenorhabditis elegans
check_url/1616?article_type=v

Modeling Age-Associated Neurodegenerative Diseases in Caenorhabditis elegans

Read Article