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Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions
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Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions

Using Caenorhabditis elegans to Screen for Tissue-Specific Chaperone Interactions

DOI:
10.3791/61140-v

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06:55 min

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June 07, 2020

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, , ,
1Department of Life Sciences,Ben-Gurion University of the Negev

Chapters

  • 00:05Introduction
  • 00:30Non‐Stressful Embryo Synchronization
  • 02:06Common Phenotypic Assays: Animal Cultivation, Embryonic Lethality, and Paralysis
  • 03:30Protein Knockdown Validation
  • 05:02Results: Representative Tissue‐Specific Chaperone Interaction Screening
  • 06:26Conclusion

Summary

Automatic Translation

Automatic Translation

To study chaperone-chaperone and chaperone-substrate interactions, we perform synthetic interaction screens in Caenorhabditis elegans using RNA interference in combination with mild mutations or over-expression of chaperones and monitor tissue-specific protein dysfunction at the organismal level.

Tags

Keywords: Caenorhabditis ElegansEmbryo SynchronizationM9 BufferNGM PlateCell ScraperCentrifugationSynchronized NematodesRNAiEmbryonic Lethality

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