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Isolering af normale og cancer-associeret fibroblaster fra friske væv ved fluorescens-aktiveret cellesortering (FACS)
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Isolering af normale og cancer-associeret fibroblaster fra friske væv ved fluorescens-aktiveret cellesortering (FACS)

Article DOI: 10.3791/4425-v • 11:31 min • January 14th, 2013
January 14th, 2013 •
1, 1, 1, 1, 1
1Department of Pathology, Sackler School of Medicine, Tel Aviv University

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Cancer associeret Fibroblaster (CAFS) lette tumor initiering, vækst og progression gennem signalere, der fremmer spredning, angiogenese og inflammation. Her beskriver vi en fremgangsmåde til at isolere rene populationer af normale fibroblaster og CAFS fra frisk muse og humane væv ved cellesortering under anvendelse PDGFRα som overflademarkør.

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Cancer Biology Cellular Biology molekylærbiologi medicin onkologi patologi Bioengineering Biomedical Engineering Kræft-associerede fibroblaster fibroblast FACS sortering PDGFRalpha Brystkræft hudkræft stroma tumor cancer væv celler kultur menneske mus dyremodel
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