Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal

A subscription to JoVE is required to view this content.
You will only be able to see the first 2 minutes.

Ultrasonic-augmented Primary Adult Fibroblast Isolation

Ultrasonic-augmented Primary Adult Fibroblast Isolation

Article DOI: 10.3791/59858-v
July 29th, 2019


Summary July 29th, 2019

We present a protocol to isolate primary adult fibroblasts in an easy, fast and reliable way, performable by beginners (e.g., students). The procedure combines enzymatic tissue digestion and mechanical agitation with ultrasonic waves to obtain primary fibroblasts. The protocol can easily be adapted to specific experimental requirements (e.g., human tissue).


Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter