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5/6th Nephrectomy in Combination with High Salt Diet and Nitric Oxide Synthase Inhibition to Induce Chronic Kidney Disease in the Lewis Rat
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5/6th Nephrectomy in Combination with High Salt Diet and Nitric Oxide Synthase Inhibition to Induce Chronic Kidney Disease in the Lewis Rat

5/6th Nephrectomy in Combination with High Salt Diet and Nitric Oxide Synthase Inhibition to Induce Chronic Kidney Disease in the Lewis Rat

DOI:
10.3791/50398-v

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08:50 min

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July 03, 2013

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, , ,
1Department of Nephrology & Hypertension,University Medical Center Utrecht

Chapters

  • 00:05Title
  • 01:15Preparation for Surgery
  • 05:13Left Side Subtotal Nephrectomy
  • 06:12Representative Nephrectomy Results
  • 08:23Conclusion

Summary

Automatic Translation

Automatic Translation

A two-stage method to establish chronic kidney disease (CKD) in the Lewis rat by surgically removing 5/6th of renal mass is described. Combination of the surgical procedure, NOS-inhibition and a high-salt diet leads to a model resembling human CKD, allowing study of causal mechanisms and development of novel therapeutic interventions.

Tags

Keywords: 5/6th NephrectomyChronic Kidney DiseaseLewis RatHigh Salt DietNitric Oxide Synthase InhibitionGlomerular Filtration RateRenal Plasma FlowHypertensionProteinuriaUremiaTubulo-interstitial DamageGlomerulosclerosis

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