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Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation
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Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation

Platelet-based Detection of Nitric Oxide in Blood by Measuring VASP Phosphorylation

DOI:
10.3791/58647-v

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07:13 min

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January 07, 2019

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, ,
1Department of Pharmacology, Faculty of Dentistry,Mahidol University, 2Molecular Medicine Branch, National Institute of Diabetes and Digestive and Kidney Diseases,National Institutes of Health

Chapters

  • 00:04Title
  • 00:45Blood Sample Preparation and Deoxygenation
  • 02:52Red Blood Cell (RBC) Nitrite Reduction and Vasodilator-stimulated Phosphoprotein (VASP) Western Blotting
  • 04:39Results: Representative Platelet-based Nitric Oxide (NO) Detection
  • 06:18Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we present a protocol to address the potential use of platelets as a highly sensitive nitric oxide sensor in blood. It describes initial platelet preparation and the use of nitrite and deoxygenated red blood cells as nitric oxide generators.

Tags

Keywords: PlateletNitric OxideVASP PhosphorylationBloodIn VitroIn VivoP-VASPSensorCentrifugationPlatelet-rich PlasmaTyrode BufferRed Blood CellsDeoxygenationNitrite Reduction

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