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Neuroscience

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Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture
 

Laser Capture Microdissection of Neurons from Differentiated Human Neuroprogenitor Cells in Culture

Article doi: 10.3791/50487
September 16th, 2013

Summary September 16th, 2013

Human neuroprogenitor cells (NPCs) were expanded under proliferating conditions. NPCs were differentiated into neuron-rich cultures in the presence of a combination of neurotrophins. Neuronal markers were detected by immunofluorescence staining. To isolate a pure population of neurons, NPCs were differentiated on PEN membrane slides and laser capture microdissection was performed.

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