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Laser Capture Microdissection – A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Laser Capture Microdissection – A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area

Laser Capture Microdissection – A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area

DOI:
10.3791/52336-v

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08:29 min

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February 06, 2015

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, ,
1Department of Basic Sciences,Mississippi State University College of Veterinary Medicine

Chapters

  • 00:05Title
  • 01:12Preparaion of Slides and Tissue for Laser Capture Microscopy
  • 03:08Laser Capture Microdissection System
  • 05:19Capturing the Ventral Tegmental Area from PEN Membrane Slides
  • 06:19Results: Dopamine Neuron Isolation Using Laser Capture Microdissection
  • 08:05Conclusion

Summary

Automatic Translation

Automatic Translation

The isolation of individual dopamine neurons or the ventral tegmental area with direct or indirect immunohistochemistry is demonstrated using laser capture microdissection. Parameters for isolation of tissue from a glass slide using an infrared laser and from membrane slides using the combination of an infrared and ultraviolet laser are discussed.

Tags

Keywords: Laser Capture MicrodissectionLCMDopamine NeuronsVentral Tegmental AreaTyrosine HydroxylaseImmunohistochemistryInfrared LaserUV LaserPEN MembraneRNA ExtractionQuantitative PCR

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