High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning

Angela Kolodziej; Karl-Heinz Smalla; Sandra Richter; Alexander Engler; Rainer Pielot; Daniela C. Dieterich; Wolfgang Tischmeyer; Michael Naumann; Thilo K&#228;hne

doi:10.3791/54992

Journal

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Neuroscience

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High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning

10,530 Views

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10:36 min

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December 15, 2016

DOI:

10.3791/54992-v

DOI:

10.3791/54992-v

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10:36 min

December 15, 2016

•

10517 Views

Angela Kolodziej¹, Karl-Heinz Smalla¹, Sandra Richter², Alexander Engler¹, Rainer Pielot^1,3, Daniela C. Dieterich³, Wolfgang Tischmeyer¹, Michael Naumann², Thilo Kähne²

¹Leibniz Institute for Neurobiology (LIN)²Institute of Experimental Internal Medicine,Medical School, Otto von Guericke University Magdeburg ³Institute of Pharmacology and Toxicology,Medical School, Otto von Guericke University

Summary

The identification of molecules and pathways controlling synaptic plasticity and memory is still a major challenge in neuroscience. Here, a workflow is described addressing the relative quantification of synaptic proteins supposedly involved in the molecular reorganization of synapses during learning and memory consolidation in an auditory learning paradigm.