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High Resolution Quantitative Synaptic Proteome Profiling of Mouse Brain Regions After Auditory Discrimination Learning
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Chapters
- 00:05Title
- 01:14Auditory Discrimination Learning in the Shuttle Box (FMTD Paradigm)
- 02:28Brain Dissection
- 03:43Purification of Synaptosomes from Crude Brain Membrane Fractions
- 06:29Sample Preparation for Mass Spectrometry Using In-solution Digest
- 09:02Results: Synaptic Abundance of selected Proteins After FMTD Task Training
- 09:53Conclusion
The identification of molecules and pathways controlling synaptic plasticity and memory is still a major challenge in neuroscience. Here, a workflow is described addressing the relative quantification of synaptic proteins supposedly involved in the molecular reorganization of synapses during learning and memory consolidation in an auditory learning paradigm.
